SYK (Phospho-Tyr323)Fluorometric Cell-Based ELISA Kit

Manufactured by Assay Genie, this fluorometric cell-based ELISA kit is designed for the qualitative detection of SYK phosphorylation at tyrosine 323 in cultured cells. The assay uses a lysate-free, indirect ELISA format in which phospho-SYK (Tyr323)-specific primary antibodies are captured and visualised via dye-conjugated secondary antibodies, enabling fluorometric readout without cell lysis.

It allows researchers to monitor SYK phosphorylation and expression profiles, to measure the relative amounts of phosphorylated SYK (Tyr323), and to screen the effects that various treatments, inhibitors (such as siRNA or chemicals) or activators have on SYK phosphorylation. Two normalisation strategies are built into the workflow: a monoclonal GAPDH antibody is included as an internal positive control, and an antibody against the non-phosphorylated counterpart of SYK (Tyr323) is provided so that RFU values for phospho-SYK can be normalised against total SYK.

The kit is reactive with human, mouse and rat samples. It has a stated dynamic range of > 5000 Cells and is intended to be read on a fluorescent plate reader at Ex/Em 651/667 (Dye 1) and 495/521 (Dye 2). Storage conditions are 4°C with 6 Months stability.