SYK (Phospho-Tyr323)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric cell-based ELISA kit provides a convenient, lysate-free, high throughput method for detecting and quantifying phosphorylated SYK protein directly in cultured cells. The assay uses an indirect ELISA format: SYK (Phospho-Tyr323) is captured by phospho-specific primary antibodies, and HRP-conjugated secondary antibodies enable colorimetric detection at 450 nm. The kit is reactive with human, mouse, and rat samples and supports a dynamic range of > 5000 Cells, making it suitable for measuring relative levels of phosphorylated SYK and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on SYK phosphorylation.

A monoclonal anti-GAPDH antibody is included to serve as an internal positive control for normalizing target absorbance values. In addition, the Crystal Violet whole-cell staining method may be applied to determine cell density, so that absorbance values can be normalized to cell amounts and plating differences adjusted.