Stathmin 1 (Phospho-Ser15)Colorimetric Cell-Based ELISA Kit

Assay Genie's Cell-Based ELISA Kit is a high-quality assay intended for the precise measurement of phosphorylated Stathmin-1 levels in cell lysates and tissue extracts. The kit targets Stathmin 1 (Phospho-Ser15), a key regulator of microtubule dynamics and cytoskeleton organization whose dysregulation has been implicated in diseases such as cancer, Alzheimer's disease, and inflammatory conditions.

The assay uses a lysate-free, high-throughput indirect ELISA format suitable for adherent cells. Stathmin 1 (Phospho-Ser15) is captured by phospho-specific primary antibodies, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction read at 450 nm. Researchers can use the kit to measure relative amounts of phosphorylated Stathmin 1 in cultured cells and to screen for the effects of various treatments, inhibitors, or activators on Stathmin 1 phosphorylation. The kit is reactive with human, mouse, and rat samples and supports a dynamic range of > 5000 Cells. A monoclonal GAPDH antibody is included as an internal positive control, and Crystal Violet staining may be used to determine cell density for absorbance normalization.

Categorized under Developmental Biology research, this phospho-specific cell-based ELISA is associated with gene symbol STMN1 (Gene ID: 3925, UniProt ID: P16949).