STAT5B Transcription Factor Activity Assay

STAT5B is a member of the STAT family activated downstream of JAK kinases in response to cytokines including IL-2, IL-7, growth hormone, and prolactin. Upon phosphorylation, STAT5B dimerizes and translocates to the nucleus where it binds gamma-activated sequence (GAS) elements to regulate genes controlling lymphocyte proliferation, NK cell maturation, and hepatic responses to growth hormone. This Assay Genie DNA-binding immunoassay provides a quantitative measure of STAT5B activation in nuclear and whole-cell lysates from human, mouse, and rat samples, offering a microplate-based alternative to EMSA for evaluating JAK-STAT pathway activity in immunology, oncology, and endocrinology research. The kit contains a pre-coated plate, specific detection reagents, and developing solutions sufficient for 96 determinations, with all components stored at 4 degrees C to preserve binding activity through the indicated shelf life. Researchers use this assay to monitor pathway activation following cytokine stimulation, screen for JAK inhibitors and small-molecule modulators, profile STAT5B activity in tumor cell lines, and compare signaling responses across different treatment conditions in a research use only workflow.