STAT5A (Phospho-Ser780)Colorimetric Cell-Based ELISA Kit

Designed for the accurate measurement of STAT5A phosphorylation at serine 780 in cell lysates, this colorimetric cell-based ELISA kit from Assay Genie provides a convenient, lysate-free, high-throughput platform for studying STAT5A signalling and expression in cells. The assay can measure relative amounts of phosphorylated STAT5A in cultured cells and is suitable for screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on STAT5A phosphorylation.

Detection is achieved by an indirect ELISA format: STAT5A (Phospho-Ser780) is captured by phospho-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction at 450 nm upon substrate addition. For normalization, a monoclonal antibody specific for human GAPDH is included as an internal positive control, and Crystal Violet whole-cell staining may be used to determine cell density.

The kit is reactive with Human, Mouse, and Rat samples and is suitable for adherent cells, with a dynamic range of > 5000 Cells. STAT5A is a transcription factor of the STAT family that is phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors; phosphorylation at serine 780 is known to regulate its activity in processes relevant to cancer, immune response, and other diseases.