STAT5A/B (Phospho-Tyr694)Colorimetric Cell-Based ELISA Kit

A Cell-Based ELISA Kit from Assay Genie designed for the detection of phosphorylated STAT5A/B at Tyr694, offering a sensitive and specific assay for use with cell lysates. The kit employs a colorimetric detection method at 450 nm and supports a dynamic range of > 5000 Cells.

Built as a convenient, lysate-free, high-throughput platform, the assay uses an indirect ELISA format in which STAT5A/B (Phospho-Tyr694)-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies catalyze the colorimetric reaction upon substrate addition. The kit shows reactivity with Human, Mouse, and Rat samples. Two normalization approaches are provided: an anti-GAPDH monoclonal antibody serves as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining method can be used to measure cell density so that absorbance values can be adjusted to cell amounts and plating differences corrected.

Phosphorylation of STAT5A/B at Tyr694 is a key event in the activation of the STAT5 signaling pathway, which plays a critical role in cell growth, differentiation, and survival, and whose dysregulation has been implicated in cancer, immune disorders, and inflammatory conditions. The kit can be used to measure relative levels of phosphorylated STAT5A/B in cultured cells and to screen for the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on STAT5A/B phosphorylation.