STAT5A/B (Phospho-Ser725/730)Colorimetric Cell-Based ELISA Kit

This Cell-Based ELISA Kit from Assay Genie is designed to measure the levels of phosphorylated STAT5A/B (Signal Transducer and Activator of Transcription 5A/B) at serine residues 725/730 in cell samples. Using a colorimetric detection method at 450 nm, the kit provides a lysate-free, high-throughput approach to analysing STAT5A/B phosphorylation and expression in cultured cells. The assay is reactive with human, mouse, and rat samples and supports a dynamic range of > 5000 cells, with adherent cells as the recommended sample type.

The indirect ELISA format captures STAT5A/B (Phospho-Ser725/730) with specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control for normalizing the target absorbance values, and the Crystal Violet whole-cell staining method can be used to determine cell density after the colorimetric measurement. The kit is suitable for measuring the relative amounts of phosphorylated STAT5A/B in cultured cells and for screening the effects that various treatments, inhibitors (such as siRNA or chemicals), or activators have on STAT5A/B phosphorylation.

Phosphorylation of STAT5A/B at Ser725/730 is a key signal transduction event that regulates important cellular processes such as cell growth, differentiation, and survival. Dysregulation of STAT5A/B phosphorylation has been implicated in various diseases, including cancer, inflammatory disorders, and autoimmune diseases, making this kit a valuable tool for studying the molecular mechanisms underlying these conditions and for discovering potential therapeutic targets.