STAT5A/B Colorimetric Cell-Based ELISA Kit

Manufactured by Assay Genie, this Cell-Based ELISA Kit is designed for the sensitive and accurate measurement of STAT5A/B levels in cell lysates and tissue extracts. STAT5A/B is a transcription factor involved in cellular processes such as cell growth, differentiation, and survival, with dysregulation linked to cancer, autoimmune disorders, and hematopoietic malignancies.

Reactive with Human, Mouse, and Rat samples, the assay uses a Colorimetric detection method read at 450 nm with a dynamic range of > 5000 Cells. The kit operates as a lysate-free, high-throughput, indirect ELISA in which STAT5A/B is captured by target-specific primary antibodies and detected via HRP-conjugated secondary antibodies. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and a Crystal Violet whole-cell staining step may be used to determine cell density.

Intended for adherent cells, the kit has storage conditions of 4°C with 6 Months stability. Database references listed include Gene ID 6777 and UniProt IDs P42229/P51692.