STAT3 Transcription Factor Activity Assay

The STAT3 Transcription Factor Activity Assay from Assay Genie quantifies activated STAT3 binding to its consensus oligonucleotide sequence using a DNA-binding ELISA approach. The microplate is pre-coated with STAT3-specific double-stranded DNA probes, enabling direct measurement of transcription factor activity from nuclear and cell lysates without radioactivity.

Validated reactivity across Human, Mouse, and Rat samples makes this kit broadly applicable to studies of the JAK-STAT signaling cascade, including cytokine signaling, oncology research, and immune regulation. The assay detects phosphorylated, transcriptionally active STAT3 that has translocated to the nucleus, providing a functional readout complementary to western blot analysis.

Each kit contains a pre-coated microplate, STAT3-specific antibodies, detection reagents, and nuclear extraction controls. Store reagents at -20°C and protect from light to preserve antibody activity. Intended for research use only in cell and molecular biology laboratories.