STAT3 (Phospho-Tyr705)Colorimetric Cell-Based ELISA Kit

A cell-based ELISA kit from Assay Genie enables qualitative detection of phosphorylated STAT3 at tyrosine 705 in cultured cells. The assay uses a colorimetric detection method measured at 450 nm in an indirect ELISA format, in which STAT3 (Phospho-Tyr705) is captured by specific primary antibodies and detected via HRP-conjugated secondary antibodies.

Described as lysate-free, high-throughput, and sensitive, the kit has a dynamic range of > 5000 cells and shows reactivity with human, mouse, and rat samples. It is suitable for adherent cells and can be used to measure relative levels of phosphorylated STAT3, as well as to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on STAT3 phosphorylation. A monoclonal GAPDH antibody is included as an internal positive control, and a Crystal Violet whole-cell staining method is provided for normalization to cell density.

STAT3 is a transcription factor involved in cellular processes such as cell growth, differentiation, and survival, and phosphorylation at tyrosine 705 is recognised as a key regulatory event in its activation, making this kit relevant to studies of signalling pathways and disease mechanisms.