STAT2 (Phospho-Tyr690)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed to detect and quantify phosphorylated STAT2 protein at Tyr690 in cell lysates, supporting research into immune regulation, antiviral responses and JAK-STAT pathway biology. The kit is described as offering high sensitivity and specificity for accurate and reproducible results in cell-based assays, and can be used to measure the relative amounts of phosphorylated STAT2 in cultured cells as well as to screen the effects of treatments, inhibitors and activators on STAT2 phosphorylation.

Reactivity is reported for human and rat samples, with a dynamic range of > 5000 Cells and a colorimetric detection at 450 nm using an indirect ELISA format. Capture relies on phospho-specific and total primary antibodies, with HRP-conjugated secondary antibodies driving the colorimetric reaction upon substrate addition. The assay is lysate-free and high throughput. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance, and a Crystal Violet whole-cell staining step is recommended to determine cell density and adjust for plating differences.

STAT2 is a member of the STAT family of transcription factors; phosphorylation at Tyr690 is required for its activation and function in the nucleus, where it regulates the expression of genes involved in antiviral responses and immune modulation. The kit is classified under the Immunology research area (Sub Type: Phospho), with UniProt entry P52630 and NCBI Gene ID 6773.