SSBP2 Colorimetric Cell-Based ELISA

From Assay Genie, this Cell-Based ELISA kit is designed for the accurate detection of SSBP2 (single-stranded DNA binding protein 2) levels in cell lysates and tissue homogenates. The assay uses a colorimetric detection method read at 450 nm and is reactive with human and mouse samples, with a dynamic range of > 5000 Cells. SSBP2 is a subunit of a single-stranded DNA-binding complex involved in the maintenance of genome stability and plays a critical role in DNA replication and repair processes, with dysregulation implicated in various diseases including cancer and neurodegenerative disorders.

The kit provides a convenient, lysate-free, high-throughput and sensitive assay that can detect SSBP2 protein expression profiles in cultured cells, and may also be used to screen the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators on SSBP2. Qualitative determination of SSBP2 concentration is achieved by an indirect ELISA format, in which SSBP2 is captured by SSBP2-specific primary antibodies and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody; the conjugated HRP then catalyses a colorimetric reaction upon substrate addition. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalisation, and a Crystal Violet whole-cell staining method may be used to determine cell density to adjust for plating differences.