Src (Phospho-Tyr418)Fluorometric Cell-Based ELISA Kit

This Fluorometric Cell-Based ELISA Kit from Assay Genie is a convenient, lysate-free, high throughput and sensitive assay designed to monitor Src phosphorylation and expression profile in cells. The kit supports measurement of the relative amounts of phosphorylated Src (Tyr418) in cultured cells and can be used to screen the effects of various treatments, inhibitors such as siRNA or chemicals, or activators on Src phosphorylation.

Qualitative determination of Src (Phospho-Tyr418) concentration is achieved through an indirect ELISA format, in which Src (Phospho-Tyr418)-specific primary antibodies capture the target while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling fluorometric detection. Two normalization strategies are provided: a monoclonal antibody specific for human GAPDH acts as an internal positive control for normalizing target RFU values, and an antibody against the nonphosphorylated counterpart of Src (Phospho-Tyr418) is included for normalizing phosphorylated Src RFU values.

Specifications include a dynamic range of > 5000 Cells, fluorometric detection, storage at 4°C for 6 Months, and reactivity with Human, Mouse, and Rat samples. The kit targets Phospho-Src (Tyr418) with the SRC gene symbol, referencing Gene ID: 6714, UniProt ID: P12931, OMIM #: 190090, and Unigene #: Hs.195659. Fluorescent plate reading is performed at Ex/Em of 651/667 (Dye 1) and 495/521 (Dye 2).