SP3/4 Transcription Factor Activity Assay

This DNA-binding ELISA from Assay Genie quantifies the GC-box binding activity of SP3 and SP4 transcription factors using a microplate format.

Each kit contains pre-coated wells with consensus SP-binding oligonucleotide sequences; active SP3 and SP4 in nuclear or whole-cell lysate samples bind these sequences and are detected with isoform-specific antibodies and a chromogenic substrate read on a standard plate reader.

Validated for human, mouse, and rat samples, requiring only a small sample volume in the microliter range for nuclear extracts.

Common research applications include profiling changes in SP3/SP4 activity during differentiation, hypoxia, drug treatment, or gene-knockdown experiments, and comparing transcription factor binding across tissues or cell lines.

Reagents should be stored refrigerated; working antibody solutions should be aliquoted to avoid repeated freeze-thaw cycles. For research use only; not intended for diagnostic or therapeutic procedures.