SP2 Transcription Factor Activity Assay

The SP2 Transcription Factor Activity Assay from Assay Genie is a DNA-Binding ELISA that measures the functional DNA-binding activity of the Sp2 transcription factor in nuclear and cell lysates. Sp2 belongs to the Sp/KLF family of zinc finger proteins and specifically recognizes GC-box motifs and other GC-rich promoter elements, where it can act as either an activator or a repressor depending on cellular context and co-factor recruitment.

This microplate kit contains 96 assays and has been validated for use with human, mouse, and rat samples, supporting cross-species comparisons in gene regulation studies. All components should be stored at -20 °C with freeze-thaw cycles minimized to preserve the binding activity of the capture probes and detection reagents. Researchers commonly apply this kit to monitor changes in Sp2 activity following siRNA knockdown, growth factor stimulation, or pharmacological treatment, and the resulting data complements chromatin immunoprecipitation experiments by quantifying the functionally DNA-bound fraction of Sp2 in the nuclear compartment.