SP2 Colorimetric Cell-Based ELISA

From Assay Genie, this cell-based ELISA kit enables colorimetric detection of SP2 protein expression in cultured cells, with absorbance measured at 450 nm. Reactive against human and mouse samples, the assay operates as a lysate-free, high-throughput format suited to studying cell signaling pathways, protein expression, and drug efficacy. It provides a dynamic range of > 5000 cells. SP2 is a transcription factor of the Sp1 C2H2-type zinc-finger protein family that binds GC box promoter elements and selectively activates mRNA synthesis.

The assay uses an indirect ELISA format: SP2 is captured by SP2-specific primary antibodies, while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Substrate addition produces a colorimetric reaction. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet whole-cell staining can be used to adjust absorbance to cell density and correct for plating differences.

Intended for research use only, the kit is applicable to epigenetics and nuclear signaling research. It can be used to measure relative SP2 levels in cultured cells and to screen the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on SP2 expression.