SOX9 Colorimetric Cell-Based ELISA Kit

From Assay Genie, this In-Cell ELISA is designed for detecting and quantifying SOX9 protein levels in cell lysates and tissue extracts. Provided in a 96-well microplate format with colorimetric detection at 450 nm, the assay is reactive in human and mouse samples and has a dynamic range of > 5000 cells.

The cell-based format is lysate-free and offers a high-throughput, sensitive method for measuring the relative amount of SOX9 in cultured adherent cells, as well as screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on SOX9 expression. Qualitative determination is achieved through an indirect ELISA format, in which SOX9-specific primary antibodies capture the target while HRP-conjugated secondary antibodies bind the Fc region of the primary antibody and catalyse a colorimetric reaction upon substrate addition.

Because the assay is qualitative, normalisation methods are supplied: a monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining can be used to determine cell density so absorbance values can be adjusted for plating differences. The kit is stored at 4°C and is stable for 6 months. SOX9 is a key transcription factor involved in chondrogenesis and cartilage formation, and its dysregulation has been implicated in skeletal disorders and certain cancers, making this kit suited to research in cell biology, developmental biology, and disease mechanisms.