SOX8/9/17/18 Colorimetric Cell-Based ELISA

From Assay Genie, this cell-based ELISA kit is designed to detect SOX8, SOX9, SOX17, and SOX18 in cell lysates, tissue homogenates, and other biological samples. It is reactive with human and mouse samples, uses a colorimetric detection method read at 450 nm, and is suited to the research area of Epigenetics and Nuclear Signaling. The intended sample type is adherent cells.

The assay is lysate-free and uses an indirect ELISA format: SOX8/9/17/18-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition. It can be used to measure the relative amounts of SOX8/9/17/18 in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on these proteins.

A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalising target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density so that absorbance values can be normalised to cell amounts. The kit has a dynamic range of > 5000 Cells and is stored at 4°C with a stated stability of 6 Months.