SOX6 Transcription Factor Activity Assay

The Assay Genie SOX6 kit quantifies DNA-binding activity of the SOX6 transcription factor, a member of the SOX-D family that governs chondrogenesis, skeletal muscle differentiation, and erythropoiesis. The microplate is pre-coated with a specific double-stranded DNA oligonucleotide containing the consensus SOX6 binding motif, allowing nuclear and cell lysates to be added directly. Active SOX6 in the sample binds the immobilized probe and is detected with an enzyme-linked antibody system, providing a quantitative readout of transcriptional regulatory activity rather than total protein abundance. The kit is configured for 96 Assays and is reactive with Human, Mouse, and Rat samples, supporting studies in developmental biology, stem cell research, and disease models where SOX6 dysregulation is implicated. Store reagents refrigerated at 4 degrees C and avoid repeated freeze-thaw cycles of the lysate controls to preserve binding activity.