SOX-9 (Phospho-Ser181)Transcription Factor Activity Assay

The Assay Genie SOX-9 (Phospho-Ser181) Transcription Factor Activity Assay is a DNA-binding ELISA built around a microplate pre-coated with a double-stranded oligonucleotide bearing the SOX-9 consensus recognition sequence. The kit quantifies the DNA-binding competence of SOX-9 that has been phosphorylated at serine 181, a PKA-driven modification that potentiates SOX-9 transcriptional output during chondrogenesis and cartilage matrix gene activation.

Researchers working on skeletal development, sex determination pathways, or chondrocyte differentiation use this colorimetric assay to probe activated SOX-9 from human, mouse, and rat nuclear and cell lysates, with reactivity validated across these three species. The plate format supports 96 assays, and components are shipped refrigerated and should be stored at -20°C between uses to preserve phospho-epitope binding reagents. All reagents are intended for research use only, in vitro applications.