SNAI1 (Phospho-Ser246)Fluorometric Cell-Based ELISA Kit

Assay Genie offers a fluorometric cell-based phospho ELISA kit designed to monitor phosphorylation of SNAI1 at Ser246 and to profile SNAI1 expression in cells. The assay is described as a convenient, lysate-free, high-throughput, and sensitive kit that uses an indirect ELISA format, in which target-specific primary antibodies capture phosphorylated SNAI1 (Ser246) and Dye 1- and Dye 2-conjugated secondary antibodies allow fluorometric readout. The kit is intended for measuring the relative amounts of phosphorylated SNAI1 in cultured cells and for screening the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on SNAI1 phosphorylation.

The assay is reactive with human, mouse, and rat samples. A monoclonal antibody specific for human GAPDH is included as an internal positive control to normalize target RFU values, and an antibody against the non-phosphorylated counterpart of SNAI1 (Ser246) is provided for further normalization. The dynamic range is greater than 5000 cells. Recommended storage is at 4°C with a stated stability of 6 months. The kit is supplied in a 96-well black cell culture clear-bottom microplate format, and the target is designated Phospho-SNAI1 (Ser246). Database identifiers associated with SNAI1 include Gene ID 6615 and UniProt ID O95863.