SNAI1 (Phospho-Ser246)Colorimetric Cell-Based ELISA Kit

Assay Genie's cell-based ELISA kit is designed to detect phosphorylated SNAI1 at serine 246 in cultured cells, providing a lysate-free, high-throughput method for studying this EMT-associated transcription factor. The assay uses an indirect ELISA format in which SNAI1 (Phospho-Ser246) is captured by phospho-specific primary antibodies and detected via HRP-conjugated secondary antibodies that catalyze a colorimetric reaction upon substrate addition.

The kit is reactive with human and mouse samples and has a stated dynamic range of > 5000 cells, with absorbance read at 450 nm. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining can be used to determine cell density, allowing results to be normalised to cell amounts. SNAI1 phosphorylation at Ser246 has been shown to modulate its activity, and the kit is intended for research into epithelial-mesenchymal transition, metastasis, and related cellular processes where SNAI1 and its phosphorylation status play a role.