Smad3 (Phospho-Thr179)Colorimetric Cell-Based ELISA Kit

A phospho-specific cell-based ELISA kit from Assay Genie is designed to detect SMAD3 phosphorylation at threonine 179 in cultured cells. The kit enables qualitative measurement of phosphorylated Smad3 levels using an indirect ELISA format, in which Smad3 (Phospho-Thr179)-specific primary antibodies capture the target and HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition.

Reactivity is reported for human, mouse and rat samples, with adherent cells as the indicated sample type. The assay uses a microplate-based format with colorimetric detection at 450 nm and a dynamic range of > 5000 cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance values, and Crystal Violet staining can be used to determine cell density after the HRP colorimetric measurement, allowing absorbance values to be normalized to cell number.

SMAD3 functions as a key signaling molecule in the TGF-β pathway, regulating cell growth, differentiation, and apoptosis. Phosphorylation at threonine 179 is a critical event in pathway activation, making the kit relevant to studies of signal transduction, drug screening and biomarker discovery related to TGF-β signaling.