Smad3 (Phospho-Ser204)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed to detect phosphorylated Smad3 (Phospho-Ser204) levels in cell cultures. It is a convenient, lysate-free, high-throughput and sensitive assay for measuring Smad3 protein phosphorylation and expression profiles in cells, and is suited to assessing the relative amounts of phosphorylated Smad3 in cultured cells as well as screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on Smad3 phosphorylation. The sample type is adherent cells.

Qualitative determination is achieved through an indirect ELISA format, in which target-specific primary antibodies capture Smad3 (Phospho-Ser204) and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling the HRP enzyme to catalyse a colorimetric reaction upon substrate addition that is read at 450 nm. The kit is reactive with Human, Mouse and Rat samples and operates with a dynamic range of > 5000 Cells. A monoclonal anti-GAPDH antibody is included as an internal positive control, and a Crystal Violet whole-cell staining method can be used to normalise absorbance readings to cell density. Smad3 is a key protein in the TGF-beta signalling pathway, and phosphorylation at Ser204 is known to be important for its activation and function in processes that regulate cell growth, differentiation and development. The sub type is Phospho, and the kit falls within the Immunology research area (Gene ID: 4088; UniProt ID: P84022; molecular weight 25,722 Da).