Smad1 (Phospho-Ser187)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit targets Smad1 phosphorylation at Serine 187, supporting qualitative measurement of Smad1 phosphorylation and expression in cultured cells. The kit can be used to screen the effects of treatments, inhibitors, or activators on Smad1 phosphorylation.

The assay uses an indirect ELISA format. Target-specific primary antibodies capture Smad1 (Phospho-Ser187), and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction at 450 nm. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance. Crystal Violet whole-cell staining may be used to determine cell density and adjust for plating differences. Reactivity is reported for human and mouse samples, the sample type is adherent cells, and the dynamic range is > 5000 Cells.

SMAD1 is a transcription factor in the TGF-beta/BMP signaling pathway, phosphorylated and activated by bone morphogenetic protein type 1 receptor kinases. Phosphorylation at Serine 187 is required for activation and downstream signaling, and the kit is therefore relevant to studies of cell growth, differentiation, apoptosis, and related disease processes.