SLP-76 (Phospho-Tyr128)Fluorometric Cell-Based ELISA Kit

Manufactured by Assay Genie, this Fluorometric Cell-Based ELISA Kit enables qualitative detection of SLP-76 phosphorylated at tyrosine 128 in cultured cells. It uses a lysate-free, indirect ELISA workflow in which target-specific primary antibodies are bound by Dye-1 and Dye-2 conjugated secondary antibodies, allowing fluorometric read-out on a dual-channel plate reader.

The kit is intended for monitoring SLP-76 phosphorylation and expression profiles in cells, and can be used to assess the effects of treatments, inhibitors (e.g. siRNA or chemicals) or activators on SLP-76 phosphorylation. A monoclonal anti-GAPDH antibody is included as an internal positive control for RFU normalization, and an antibody against the non-phosphorylated counterpart of SLP-76 is provided so that phosphorylated signals can be normalized to total SLP-76 levels. The stated dynamic range is > 5000 cells, with reactivity reported for human, mouse and rat samples. Storage is at 4 °C with a stated stability of 6 months.

Read-out is performed at Ex/Em 651/667 nm (Dye 1) and 495/521 nm (Dye 2). The corresponding target is the LCP2 gene product (Gene ID 3937; UniProt Q13094), an adaptor protein implicated in T cell receptor and platelet signalling.