SHP-2 (Phospho-Tyr580)Fluorometric Cell-Based ELISA Kit

From Assay Genie, this Fluorometric Cell-Based ELISA Kit is designed to monitor phosphorylation of SHP-2 at tyrosine 580 (Phospho-SHP-2 (Tyr580)) and its expression profile in cultured cells. The kit is described as convenient, lysate-free, high throughput and sensitive. It uses an indirect ELISA format in which target-specific primary antibodies are detected by Dye 1- and Dye 2-conjugated secondary antibodies, enabling fluorometric detection. The kit is intended for measuring relative levels of phosphorylated SHP-2 in cultured cells and for screening the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators on SHP-2 phosphorylation.

Reactivity covers Human, Mouse and Rat, and the assay is run as a Cell-Based ELISA with a stated dynamic range of > 5000 Cells. Storage is specified at 4°C for 6 Months. Two normalization strategies are built in: a monoclonal GAPDH antibody serves as an internal positive control for target RFU values, and an antibody against the non-phosphorylated counterpart of SHP-2 is included so that the RFU value for phosphorylated SHP-2 can be normalized against total SHP-2. Plates are read on a fluorescent plate reader at Ex/Em 651/667 (Dye 1) and 495/521 (Dye 2). The target corresponds to the gene PTPN11 (Gene ID: 5781; UniProt ID: Q06124).