SHP-2 (Phospho-Tyr542)Colorimetric Cell-Based ELISA Kit

A Cell-Based ELISA Kit from Assay Genie designed for the detection of phosphorylation of SHP-2 at tyrosine 542 in cultured cells. The kit provides a lysate-free, high-throughput assay for measuring the relative amounts of phosphorylated SHP-2 and for screening the effects that different treatments, inhibitors (such as siRNA or chemicals) or activators have on SHP-2 phosphorylation.

Detection is colorimetric at 450 nm using an indirect ELISA format, in which SHP-2 (Phospho-Tyr542) is captured by phospho-specific primary antibodies and HRP-conjugated secondary antibodies catalyse a colour reaction upon substrate addition. The assay is reactive with Human, Mouse and Rat samples, with a dynamic range of > 5000 cells, and is suited to adherent cell preparations. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance, and a Crystal Violet whole-cell stain may be used afterwards to measure cell number for further normalisation of plating differences. The kit is intended for research applications in cell signalling and in conditions such as cancer, inflammatory disorders and metabolic disease, where phosphorylation of SHP-2 (gene symbol PTPN11, UniProt Q06124) at tyrosine 542 influences cell growth, differentiation and migration.