SHP-1 (Phospho-Tyr564)Fluorometric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit is designed to monitor the phosphorylation and expression profile of SHP-1 (Phospho-Tyr564) in cultured cells. The kit provides a lysate-free, high throughput and sensitive approach for measuring relative amounts of phosphorylated SHP-1 (Phospho-Tyr564) and can be used to screen the effects of various treatments, inhibitors such as siRNA or chemicals, or activators on SHP-1 phosphorylation.

The assay uses an indirect ELISA format. The target is captured by SHP-1 (Phospho-Tyr564)-specific primary antibodies, while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody, enabling fluorometric detection. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalizing target RFU values, and an antibody against the non-phosphorylated counterpart of SHP-1 (Phospho-Tyr564) is provided for normalization purposes.

Key specifications include a dynamic range of > 5000 Cells, fluorometric detection read at Ex/Em: 651/667 (Dye 1) and 495/521 (Dye 2), and reactivity with human, mouse, and rat samples. The target gene is PTPN6 (Gene ID: 5777, UniProt ID: P29350). The kit is intended for cell-based phospho-ELISA applications and is stored at 4 °C with a stated stability of 6 months.