SHP-1 (Phospho-Tyr536)Fluorometric Cell-Based ELISA Kit

Manufactured by Assay Genie, this Fluorometric Cell-Based ELISA Kit is designed to monitor SHP-1 (Phospho-Tyr536) protein phosphorylation and expression profile in cells. The assay is described as convenient, lysate-free, high-throughput, and sensitive, and enables qualitative determination of phosphorylated SHP-1 in cultured cells via an indirect ELISA format. It is suited to screening the effects of various treatments, inhibitors such as siRNA or chemicals, and activators on SHP-1 phosphorylation.

Reactivity is reported for Human, Mouse, and Rat samples, with a stated dynamic range of > 5000 Cells. The fluorometric detection relies on dye-conjugated secondary antibodies read at Ex/Em 651/667 nm and 495/521 nm. An anti-human GAPDH monoclonal antibody is included as an internal positive control for normalising target RFU values, and an antibody against the non-phosphorylated counterpart of SHP-1 (Tyr536) is provided for additional normalisation. Recommended storage is 4°C with a stated stability of 6 months.

The target is Phospho-SHP-1 (Tyr536), associated with NCBI Gene Symbol PTPN6 (Gene ID 5777), UniProt ID P29350, and OMIM #176883.