Sheep S100B (S100 Calcium Binding Protein B) ELISA Kit (SHFI00066)

The Assay Genie quantitative immunoassay provides researchers with a reliable tool for measuring S100 calcium-binding protein B levels in ovine research specimens. This 96 Assays kit employs a microplate-based colorimetric readout, where absorbance values correlate with S100B concentration in the loaded sample. Each kit is configured for high-throughput laboratory workflows and includes pre-coated plate wells, detection antibodies, and standard calibrators required for constructing a calibration curve.

S100B is a well-characterized calcium-binding protein of the EF-hand family, expressed primarily in astrocytes and a subset of additional cell types within the central nervous system. The protein has been investigated extensively as a circulating biomarker in traumatic brain injury, melanoma progression, and various inflammatory disease models. In ovine research, S100B quantification supports neuroscience investigations, veterinary translational studies, and large-animal models used to bridge rodent findings and human clinical research.

Researchers should follow the enclosed protocol for specimen handling, dilution series, incubation periods, and wash steps prior to absorbance measurement. The microplate layout accommodates parallel processing of standards and unknowns, enabling efficient batch analyses in laboratory workflows focused on brain-injury biomarkers, neuroinflammation, and related signalling pathways.