Sheep IL-8 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of Interleukin-8 (IL-8) in sheep samples. The assay uses a double-antibody sandwich format with colorimetric detection, delivering a sensitivity of 9.375 pg/mL across a working range of 15.625–1000 pg/mL.

Validated sample types include serum, plasma, cell culture supernatant, and cell or tissue lysate. The kit should be stored at 2–8°C for up to 12 months. Reported linearity spans 80–105% across serum, EDTA plasma, and heparin plasma at dilutions of 1:2, 1:4, and 1:8 (n=5), while recovery ranges from 86–105% with averages between 93–96% depending on the sample matrix.

The workflow involves reagent equilibration, primary incubation at 37°C for 90 minutes, biotinylated detection antibody incubation for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB colour development in the dark for 10–20 minutes, and absorbance reading at 450 nm. Interleukin-8 is a key inflammatory cytokine involved in neutrophil recruitment, making this kit a useful tool for research into immune and inflammatory processes.