Sheep IL-1 beta DIY ELISA Kit

For ovine immunology and inflammation research, this kit from Assay Genie quantifies interleukin-1 beta (IL-1β), a key pro-inflammatory cytokine involved in host defense, fever regulation, and acute phase responses in sheep models. The format uses biotinylated detection antibody with streptavidin-HRP signal amplification and TMB substrate, supporting measurement of blood-derived samples and cell culture supernatant. Reagents are supplied for up to 10 microplate runs, with pre-coated plates requiring overnight blocking before sample addition.

Store unopened components refrigerated, and aliquot reconstituted standards for freezing at -20 °C to preserve stability across repeated use. Each kit contains wash buffer, coating buffer, substrate, and stop solution sufficient for the full format, with the DIY configuration allowing researchers to optimize incubation times for their specific tissue or fluid type. The standard curve range and sensitivity specifications are provided in the accompanying protocol insert, ensuring reproducible quantification across experimental batches.