SF1 (Phospho-Ser82)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Colorimetric Cell-Based ELISA Kit is designed for the precise measurement of SF1 (Splicing Factor 1) phosphorylation at serine 82 in cell lysates. The assay uses a lysate-free, high-throughput, indirect ELISA format, in which a phospho-specific primary antibody captures the target and an HRP-conjugated secondary antibody catalyses a colorimetric reaction read at 450 nm.

SF1, also known as splicing factor 1, plays a crucial role in alternative splicing, gene regulation, and RNA processing. Phosphorylation at serine 82 has been linked to various cellular processes and disease pathways, making it a valuable target for studying signal transduction and potential therapeutic interventions. The kit can be used to measure relative amounts of phosphorylated SF1 in cultured cells and to screen the effects of treatments, inhibitors (such as siRNA or chemicals), or activators on SF1 phosphorylation.

Reactivity is reported for human and mouse samples, with a dynamic range of > 5000 cells. The assay is suitable for adherent cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and the Crystal Violet whole-cell staining method may be used to determine cell density. This phospho-specific kit falls within the Epigenetics and Nuclear Signaling research area.