SEPT1 Colorimetric Cell-Based ELISA

This cell-based ELISA from Assay Genie provides qualitative detection of SEPT1 protein expression in cultured cells using an indirect ELISA format. Target-specific primary antibodies are captured and detected with HRP-conjugated secondary antibodies, which catalyze a colorimetric reaction upon substrate addition. The kit is lysate-free and designed for high-throughput screening of relative SEPT1 levels in adherent cells, and for evaluating the effects of treatments, inhibitors (such as siRNA or chemicals), or activators.

The assay is read at 450 nm, with a dynamic range of > 5000 cells and reactivity against human, mouse, and rat samples. It is supplied in a 96-well microplate format and stored at 4°C with 6 months stability. An anti-GAPDH monoclonal antibody is included as an internal positive control, and Crystal Violet whole-cell staining can be used to normalize absorbance values to cell density. Sample type is adherent cells.

SEPT1 is a filament-forming cytoskeletal GTPase of the septin family, implicated in cytokinesis, cytoskeletal organization, and membrane dynamics. The protein has a reported molecular weight of 18,354 Da and is encoded at chromosomal location 16p11.1 (Gene ID: 1731; UniProt ID: Q8WYJ6). The kit components include 96-well cell culture clear-bottom microplates, 10X TBS, Quenching Buffer, Blocking Buffer, 15X Wash Buffer, Primary Antibody Diluent, 100x Anti-Phospho Target Antibody, 100x Anti-Target Antibody, Anti-GAPDH Antibody, HRP-Conjugated Anti-Rabbit IgG Antibody, HRP-Conjugated Anti-Mouse IgG Antibody, SDS Solution, Stop Solution, Ready-to-Use Substrate, Crystal Violet Solution, and Adhesive Plate Seals. A microplate reader capable of measuring absorbance at 450 nm and/or 595 nm is required but not provided.