SDZ (Sulfadiazine) ELISA Kit

Veterinary antibiotic residue monitoring programs need to consolidate specimen testing onto a single workflow, and this competitive ELISA kit from Assay Genie is configured for quantitative Sulfadiazine screening across muscle, honey, milk, water, egg, serum, and urine. By packaging one validated protocol that handles this breadth of matrices on a 96-assay microplate, the kit reduces the need for separate method development when laboratories screen for compliance with veterinary drug residue limits, since Sulfadiazine is administered to livestock, poultry, and bees and residue carryover can appear in meat, dairy, honey, drinking water, and farm wastewater.

The assay uses pre-coated coupling antigen on the plate wells; sample extract and HRP-conjugated antibody compete for binding, and a TMB substrate step produces a colorimetric readout whose intensity is inversely proportional to Sulfadiazine concentration. The microplate configuration allows laboratories to run partial plates without wasting unused wells when sample throughput is low. A liquid standard curve, HRP conjugate, antibody working solution, TMB substrate reagents A and B, stop solution, concentrated wash buffer, and reconstitution buffer are all included in the kit, supporting a complete workflow from sample extraction through final quantification.

For sample preparation, the protocol calls for a small sample volume added directly to the coated wells, which keeps consumable use modest even when screening large batches of food or environmental specimens. The kit supports quality control operations at dairy processors, honey producers, aquaculture facilities, meat packing plants, water testing laboratories, and veterinary diagnostic services. Laboratory staff can interpret results using the kit's standard curve and evaluate potential cross-reactivity from structurally similar sulfonamide compounds that may co-occur in sulfonamide-treated herds.