SARS-COV-2 Spike S1 Protein IgG Antibody ELISA Kit

From Assay Genie, this in vitro enzyme-linked immunosorbent assay (ELISA) kit is intended for the quantitative measurement of SARS-CoV-2 Spike S1 Protein IgG Antibody in serum and plasma (EDTA, citrate, heparin) samples. The kit is for laboratory research use only and is not intended for use in diagnostic or therapeutic procedures. Described as a Sandwich ELISA Kit, it uses a colorimetric detection method with HRP as the conjugate and requires a microplate reader for measurement at 450 nm.

The assay has a total run time of 3.5 hours, a reported sensitivity of 16.43 pg/mL, and a standard curve range of 39.1-2500 pg/mL. The stop solution produces a colour change from blue to yellow, and the kit includes calibration standards that allow the operator to generate a standard curve of optical density versus analyte concentration. Sample volume is 100 uL. Components include an antigen coated plate, control antibody (4000×), concentrated secondary antibody (500×), control/sample diluent (R1), secondary antibody diluent (R2), wash buffer (20×), TMB substrate, stop solution, and plate sealers. The kit should be stored at 4°C and contains small quantities of sodium azide, requiring appropriate disposal precautions.