SARS-COV-2 Spike S1 Protein Antibody ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the in vitro quantitative measurement of SARS-COV-2 Spike S1 Protein Antibody in cell culture supernatant, serum and plasma (EDTA, citrate, heparin). It is intended for laboratory research use only and is not for use in diagnostic or therapeutic procedures.

Colorimetric detection uses a biotin conjugate and is read at 450 nm with a microplate reader / spectrophotometer, with the stop solution changing colour from blue to yellow. The assay has a sensitivity of 157.2 pg/mL, a standard curve range of 391–25000 pg/mL and a total run time of 4 hours. Sample concentrations are calculated by comparing O.D. values to a standard curve generated from calibration standards assayed alongside the samples.

Kit components include a Recombinant 2019-nCoV Spike S1 Protein Coated Plate, Control Antibody (1000×), Concentrated Biotin Recombinant 2019-nCoV Spike S1 Protein (250×), Streptavidin-HRP Concentrated (100×), Control/Sample Diluent (R1), Biotin-Conjugate Protein Diluent (R2), Streptavidin-HRP Diluent (R3), 20× Wash Buffer, TMB Substrate, Stop Solution and Plate Sealers. Storage is at 4 °C. Safety guidance notes that the kit contains small quantities of sodium azide, which can form explosive metal azides in lead or copper plumbing, and that all blood components and biological materials should be handled as potentially hazardous under universal precautions.