RT2 First Strand Kit (50)

QIAGEN's RT2 First Strand Kit is designed for cDNA synthesis and genomic DNA elimination in RNA samples intended for use with RT2 Profiler PCR Arrays and RT2 lncRNA PCR Arrays. The kit includes a proprietary procedure to effectively eliminate contaminating genomic DNA from RNA samples before reverse transcription. Random hexamers and oligo-dT prime reverse transcription in an unbiased manner, and a reverse transcriptase synthesises cDNA product with optimal yield and length. A built-in external RNA control helps monitor reverse transcription efficiency and test for enzyme inhibitors.

The kit is optimised for real-time PCR-based gene expression analysis using RT2 Profiler PCR Arrays and RT2 qPCR Primer Assays, and can be successfully used with other commercially available gene expression analysis assays. The proprietary Buffer GE and sample are mixed and incubated on a thermal cycler; after addition of the enzyme and additional buffer, the sample is reverse transcribed using any thermal cycler, and the transcribed cDNA is immediately ready for downstream applications.

The kits yield results with as little as 25 ng or as much as 5 µg total RNA per reaction, with the optimal amount of starting material depending on the relative abundance of the transcripts of interest. Lower abundance transcripts require more RNA; higher abundance transcripts require less RNA. Greater amounts of input RNA yield greater numbers of positive calls in the linear dynamic range of the method.