REPLI-g WTA Single Cell Kit (96)

QIAGEN's REPLI-g WTA Single Cell Kit is designed for cDNA generation and whole transcriptome amplification from single cells. It employs a novel Phi 29 polymerase, REPLI-g SensiPhi Polymerase, alongside an optimized set of buffers and reagents, and uses Multiple Displacement Amplification (MDA) for accurate cDNA amplification. The kit supports whole transcriptome amplification from a single cell up to around 1000 cells, enabling investigation of the transcriptome from very small samples. It is intended for molecular biology applications and is not intended for the diagnosis, prevention, or treatment of disease.

Following efficient cell lysis, removal of genomic DNA, and reverse transcription of the entire RNA, the workflow generates up to 20 μg cDNA per reaction, making the amplified material highly suitable for next-generation sequencing (mRNA-Seq), gene expression arrays, or quantitative PCR analysis. Typical amplified cDNA has an approximate length of 2,000 bp up to 70,000 bp and is stable during long-term storage for biobanking of samples. The kit is shipped on dry ice and should be stored upon receipt at –15°C to –30°C, or at –65°C to –90°C for longer-term storage. Each lot is quality controlled under QIAGEN's ISO-certified Quality Management System.

Compatible sample materials include tumor cells, stem cells, sorted cells, and other small samples commonly analyzed in clinical research. The kit does not lyse cell walls and is therefore not suitable for bacterial cells or for plant or other cells with cell walls. It is also not suitable for small nucleic acids such as tRNAs and miRNAs, for severely degraded RNA, or for RNA from FFPE material or samples fixed by formaldehyde, glutaraldehyde, or other fixatives.