RT_ First Strand Kit (12)

A first-strand cDNA synthesis kit from QIAGEN designed for cDNA synthesis and genomic DNA elimination in RNA samples intended for use with RT² Profiler PCR Arrays and RT² lncRNA PCR Arrays.

The kit includes a proprietary procedure to effectively eliminate contaminating genomic DNA from RNA samples before reverse transcription. Random hexamers and oligo-dT prime reverse transcription in an unbiased manner, and a reverse transcriptase synthesizes cDNA product with optimal yield and length. A built-in external RNA control helps monitor reverse transcription efficiency and tests for enzyme inhibitors. The kit is optimized for real-time PCR-based gene expression analysis using RT² Profiler PCR Arrays and RT² qPCR Primer Assays, and can be successfully used with other commercially available gene expression analysis assays.

The proprietary Buffer GE and sample are mixed and incubated on a thermal cycler. After addition of the enzyme and additional buffer, the sample is reverse transcribed using any thermal cycler. The transcribed cDNA is immediately ready for use in downstream applications. The kit yields results with as little as 25 ng or as much as 5 µg total RNA per reaction.