RFX2 Transcription Factor Activity Assay

The RFX2 Transcription Factor Activity Assay from Assay Genie measures the DNA-binding activity of Regulatory Factor X2 (RFX2), a member of the RFX family that recognizes X-box promoter elements. RFX2 is critical for spermatogenesis, ciliogenesis, and sperm flagellum assembly, and it is widely studied in the context of male infertility and motile cilia biology. This cell-based activity assay uses nuclear and cell lysates to capture and quantify transcription factor binding, providing a quantitative alternative to electrophoretic mobility shift assays (EMSAs). The kit is reactive with human, mouse, and rat samples and is intended for research use only in studies of gene regulation, developmental biology, and reproductive biology.