Reverse Transcriptase (10000 U)

For laboratories performing first-strand cDNA synthesis from RNA templates, this QIAGEN reverse transcriptase delivers robust activity across varied template amounts and priming strategies. The enzyme is supplied as a single vial containing 10,000 units of purified recombinant protein and supports applications including RT-PCR, RT-qPCR, gene expression profiling, cDNA library construction, and 5' and 3' RACE reactions.

The enzyme works at standard reaction temperatures of 37-50 °C and is compatible with random hexamer, oligo-dT, and gene-specific primer approaches. It produces full-length cDNA from messenger RNA, total RNA, and viral RNA templates, enabling downstream workflows such as PCR amplification, sequencing, and molecular cloning.

For storage and handling, keep the enzyme frozen at -20 °C and protect from light to preserve activity through multiple freeze-thaw cycles. Reaction setup typically requires a small sample volume in the microliter range, and the 10,000-unit pack size supports hundreds of standard 20 µL reverse transcription reactions. This QIAGEN reverse transcriptase is intended for research use only and serves as a core reagent in molecular biology workflows requiring accurate RNA-to-cDNA conversion for transcript analysis.