Retinoblastoma (Phospho-Thr826)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this Cell-Based ELISA Kit enables the qualitative measurement of Retinoblastoma protein phosphorylated at Thr826 in cultured cells. The assay uses an indirect ELISA format in which phospho-specific primary antibodies capture the target, while HRP-conjugated secondary antibodies catalyze a colorimetric reaction upon substrate addition, with absorbance measured at 450 nm.

Reactive with Human, Mouse, and Rat samples, the kit is lysate-free and supports high-throughput screening of the effects of various treatments, inhibitors (such as siRNA or chemicals), and activators on Retinoblastoma phosphorylation. The dynamic range is > 5000 Cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalizing target absorbance, and a Crystal Violet whole-cell staining step is used to determine cell density so that results can be normalized to cell amounts.

The target is the RB1 gene product (UniProt P06400; Gene ID 5925), the retinoblastoma tumor suppressor protein that regulates cell proliferation by controlling progression through the G1-phase restriction point, interacting with the E2F family of transcription factors and being regulated by Cdk-mediated phosphorylation.