Ret (Phospho-Tyr1062)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric cell-based ELISA kit enables the accurate and sensitive detection of phosphorylated RET (Tyr1062) in cell lysates and tissue extracts. It provides high specificity and reproducibility for investigating RET-mediated signal transduction pathways and the role of RET phosphorylation in cell growth, survival, and differentiation. Aberrant activation of the RET pathway has been implicated in numerous cancers, supporting applications in cancer biology research and targeted therapy development.

The assay uses an indirect ELISA format in which phosphorylated RET (Tyr1062) is captured by target-specific primary antibodies, while HRP-conjugated secondary antibodies generate a colorimetric signal read at 450 nm. It is a lysate-free, high-throughput approach for measuring relative levels of phosphorylated RET in cultured cells and for screening the effects of treatments, inhibitors such as siRNA or chemicals, and activators on RET phosphorylation. Reactivity has been confirmed in human, mouse, and rat samples, with a stated dynamic range of > 5000 cells. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, and a Crystal Violet whole-cell staining step may be used to determine cell density so that absorbance values can be normalized to cell amounts.

The kit is intended for the Cell Biology research field and is provided in a microplate format designed for high-throughput workflows.