Ret (Phospho-Tyr1015)Colorimetric Cell-Based ELISA Kit

This colorimetric cell-based ELISA kit from Assay Genie is designed for the accurate and sensitive measurement of phosphorylated RET at tyrosine 1015 in cell lysates. It is optimized for cell-based assays and provides the specificity and precision needed for the analysis of Ret protein phosphorylation and expression profile in cultured cells.

The assay uses a lysate-free, high-throughput, sensitive indirect ELISA format. Ret (Phospho-Tyr1015) is captured by target-specific primary antibodies, and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody, catalysing a colorimetric reaction upon substrate addition that is read at 450 nm. The kit is suitable for measuring the relative amounts of phosphorylated Ret in cultured cells and for screening the effects of treatments, siRNA, chemical inhibitors, or activators on Ret phosphorylation, with qualitative determination of Ret (Phospho-Tyr1015) levels. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalising target absorbance values, and Crystal Violet whole-cell staining may be used to determine cell density and adjust for plating differences.

The kit is reactive with human, mouse, and rat samples, has a dynamic range of > 5000 cells, and is intended for use with adherent cells. Phosphorylation of RET at tyrosine 1015 is a critical event in RET signalling, which regulates cell growth, survival, and differentiation and has been implicated in cancer and neurodegenerative disorders, making this site an important target for studying disease mechanisms and potential therapeutic interventions.