Recoverin Colorimetric Cell-Based ELISA

Manufactured by Assay Genie, this Cell-Based ELISA kit is designed for the precise measurement of recoverin levels in cell lysates and culture supernatants. Detection is colorimetric, measured at 450 nm, and the kit operates as a lysate-free, high-throughput indirect ELISA in which recoverin is captured by specific primary antibodies and detected using HRP-conjugated secondary antibodies that catalyze a colorimetric reaction on substrate addition.

Qualitative determination of recoverin expression is achieved in cultured adherent cells, with reactivity demonstrated in Human and Mouse samples. The stated dynamic range is > 5000 Cells, the format is a 96-Well Microplate, and storage is at 4 °C with a stability of 6 Months. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, and Crystal Violet whole-cell staining can be used to determine cell density.

The kit is suited to screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on recoverin expression. Recoverin is a calcium-binding neuronal calcium sensor involved in the regulation of phototransduction in the retina, and its dysfunction has been linked to retinal disorders, making it a valuable biomarker for vision and eye disease research.