Rat Zona Pellucida Glycoprotein 2, Sperm Receptor (ZP2) ELISA Kit

Quantitative measurement of rat ZP2, a zona pellucida glycoprotein that functions as the primary sperm receptor during fertilization, supports reproductive biology studies ranging from oocyte maturation to contraceptive development. Produced by Assay Genie, this kit is configured for colorimetric readout and is sized for 96 assays; it accepts rat tissue homogenates or cell lysates as input material.

ZP2 cleavage after fertilization drives the block to polyspermy, so quantifying the native protein helps researchers characterize gamete recognition, folliculogenesis, and hormonal regulation in ovarian tissue. Each kit ships with cold pack protection and is intended for in vitro research use only workflows. Reagents require refrigerated storage and should be brought to room temperature before use, and the supplied microplate and assay consumables are single-use components. The procedure pairs a pre-coated capture antibody with a labelled detection reagent to produce a measurable color change proportional to ZP2 concentration in each sample.

Common applications include profiling ZP2 expression across rat ovarian developmental stages, comparing wild-type and genetically modified reproductive tissues, and screening experimental compounds for their effects on zona pellucida integrity. The format integrates into standard laboratory workflows applied across gamete biology, fertility research, and reproductive toxicology, where parallel protein quantification across many samples in a single run is required.