Rat Xanthine Dehydrogenase (XDH) ELISA Kit

Xanthine dehydrogenase (XDH) catalyzes the final two oxidation steps of purine catabolism, converting hypoxanthine to xanthine and then to uric acid. Its interconversion with xanthine oxidase (XOD) is a focal point in studies of oxidative stress, ischemia-reperfusion injury, and inflammatory signaling. This Assay Genie kit provides quantitative measurement of rat XDH in serum samples, plasma samples, tissue homogenates, and other rat-derived specimen types, supplied in a 96-assay microplate format configured for standard curve-based concentration determination.

Common research applications include rodent models of hyperuricemia and gout, hepatic and renal purine metabolism studies, investigation of XDH/XOD switching under hypoxic or inflammatory conditions, and preclinical screening of xanthine oxidase inhibitor compounds such as allopurinol or febuxostat analogues. The rat-specific reactivity makes this assay appropriate for translational work using Sprague-Dawley, Wistar, or other rat strains rather than mouse or human specimens, and it complements downstream readouts such as western blot or activity-based xanthine oxidase assays.

Workflow follows an absorbance-based readout on a standard microplate reader, with reagents intended for refrigerated storage and protection from light until first use to preserve antibody binding activity and the integrity of the lyophilized reference standards. Reconstituted standards and diluted samples should be handled on ice to limit freeze-thaw degradation, and each kit supports a full microplate of determinations covering the standard curve, optional controls, and unknowns run in replicate.