Rat WNT5A (Protein Wnt-5a) ELISA Kit (RTFI01430)

Wnt-5a is a secreted glycoprotein that activates non-canonical β-catenin-independent signaling pathways and influences cell migration, planar cell polarity, inflammatory cascades, and embryonic tissue patterning. The Assay Genie Rat WNT5A ELISA Kit provides a quantitative immunoassay platform for measuring Wnt-5a protein concentrations in rat research specimens.

This kit supports cancer biology research, studies of fibrotic disease, rheumatoid arthritis models, wound healing investigations, and developmental biology workflows where Wnt-5a dysregulation is implicated. Researchers apply it to investigate tumor microenvironment signaling, stem cell differentiation, and cellular communication in rodent experimental systems. The microplate format delivers 96 Assays per kit, suited to medium-throughput screening programs.

Reagents include a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, wash buffer, and substrate solution. Allow kit components to equilibrate to room temperature before running the protocol and store the kit refrigerated between uses to maintain reagent activity. For research use only; not intended for diagnostic procedures.